FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243093
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT10460.4302879967748969No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT7860.32333304537769497No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA7830.3220989497846503No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC7600.3126375502379748No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT7100.2920692903538975No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA7000.28795563837708205No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC6930.28507608199331125No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG6740.2772601432373618No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6200.25504642256255833No Hit
CTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAG6000.24681911860892744No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG5890.24229410143443045No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG5580.2295417803063025No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA5090.20938488561990679No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG4910.20198031206163894No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT4890.20115758166627584No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA4810.19786666008482348No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC4700.1933416429103265No Hit
GGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAGTAAAA4660.19169618211960032No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4440.1826461477706063No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA4420.1818234173752432No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA4330.17812113059610932No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT4310.17729840020074622No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT4290.17647566980538312No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA4270.17565293941002003No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT4170.1715392874332046No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA4160.17112792223552303No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA4100.16865973104943374No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA3810.15673014031666893No Hit
ATTATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT3710.15261648833985347No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGA3710.15261648833985347No Hit
GCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTA3690.15179375794449038No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC3620.14891420156071955No Hit
GACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCAC3550.14603464517694875No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC3510.14438918438622256No Hit
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAA3440.14150962800245173No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACT3410.1402755324094071No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC3390.139452802014044No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA3380.13904143681636247No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA3340.13739597602563627No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG3250.13369368924650238No Hit
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAA3250.13369368924650238No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC3230.13287095885113928No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAA3170.13040276766505002No Hit
GTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAG3130.12875730687432382No Hit
AGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGT3110.12793457647896073No Hit
ATATTAAATAGATATCTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTT3060.125877750490553No Hit
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTA3050.12546638529287144No Hit
GTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTT3040.1250550200951899No Hit
AGCTTATGTAGCCTTCATTCATGTTGGCAGTAAAATGCTTATTGTGTTTG3030.12464365489750835No Hit
CTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCATGGTAGTC2980.12258682890910064No Hit
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG2970.12217546371141909No Hit
GGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA2920.12011863772301136No Hit
TCCTTAGATCGTTAACAGTTACTTGGATAACTGTGGTAATTCTAGAGCTA2910.11970727252532981No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGT2900.11929590732764828No Hit
CTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCATAT2900.11929590732764828No Hit
ATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTT2890.11888454212996673No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTA2870.11806181173460363No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2840.116827716141559No Hit
TCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCA2770.11394815975778816No Hit
TTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGT2760.11353679456010662No Hit
GTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATG2740.11271406416474353No Hit
AGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAG2700.11106860337401735No Hit
GTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCT2640.10860041218792807No Hit
TCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTG2630.10818904699024653No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAA2620.10777768179256499No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGG2600.1069549513972019No Hit
ATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTC2590.10654358619952035No Hit
GTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGA2500.10284129942038645No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTA200.00694547752.59
TATAGGT200.00694547752.52
GAGATAG200.00694547752.517
GGACGTG451.6802696E-646.6666647
TACACCG404.584068E-543.755
CTTCCGA8250.043.6969681
TCCGATC8100.043.2098773
GTATATG503.469533E-642.01
TATGTCA503.469533E-642.04
CCGATCT8600.041.1046524
TAACCTT350.001253816440.06
TTCCGAT9300.038.763442
ATATGTC556.677439E-638.181823
GATCTAT556.677439E-638.181826
ATCTGAG753.522473E-837.3333327
TCTGAGC708.662446E-735.08
TAACATA400.002415694535.029
GCTCTTC2700.032.407413
TCTTCTA552.9865635E-431.81818414
CTTCTAG552.9865635E-431.81818415
Produced by FastQC (version 0.11.2)