H3W35AFXY_n02_adr3wtzt14s88.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3W35AFXY_n02_adr3wtzt14s88.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	125896
Sequences flagged as poor quality	0
Sequence length	76
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	410	0.32566562877295546	No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC	405	0.3216940967147487	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	385	0.30580796848192154	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	333	0.26450403507657116	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC	315	0.2502065196670268	No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA	294	0.2335260850225583	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	274	0.2176399567897312	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGA	15	0.0022202907	70.00001	49
CCGAGTC	25	2.3828365E-4	55.999996	15
ATTATAC	25	2.3828365E-4	55.999996	70
CCGATAG	20	0.006937734	52.5	27
TGACCGA	20	0.006937734	52.5	24
TAATGCG	30	5.859358E-4	46.666668	4
TTAATAC	35	0.001251513	40.000004	3
AAGACTC	35	0.001251513	40.000004	4
CATTATA	35	0.001251513	40.000004	69
TACTCCT	35	0.001251513	40.000004	14
CTATTCG	35	0.001251513	40.000004	29
TCTGAGC	45	9.1552734E-5	38.888885	8
CAAGGTA	45	9.1552734E-5	38.888885	4
CGGTTAA	40	0.0024112843	35.0	34
TCGGTTA	40	0.0024112843	35.0	33
AATAAGT	40	0.0024112843	35.0	54
AAGCTCG	40	0.0024112843	35.0	39
TCACGAG	45	0.0042940388	31.111109	25
ACGCCTC	45	0.0042940388	31.111109	20
CCACTGT	45	0.0042940388	31.111109	36