FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394271
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10800.27392326597695493No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA7560.19174628618386846No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT7340.1861663678028564No Hit
GTATAGGGGCTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTGGTTA7320.18565910249549167No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA6370.16156400039566693No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC5860.14862873505786628No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5690.14431697994526607No Hit
GAATAGGCCCAAGCGACTGTTTATCAAAAACACAGGTCTCTGCTAAACCG5640.14304881667685426No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA5470.13873706156425403No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT5230.13264987787587726No Hit
ATTCTAAGGTGAGCGAGCGAACTCTCGTTAAGGAACTCGGCAAAATGACC5090.12909902072432414No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC5040.1278308574559123No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT4960.12580179622645338No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA4740.12022187784544132No Hit
TTCCAGCACCGGGCAGGCGTCAGCCCCTATACATCACCTTACGGTTTAGC4570.11591012273284111No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT4390.11134473496655854No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG4330.10982293904446434No Hit
GTGCTGGAAGGTTAAGAGGAGTGGTTAGCTTCTGCGAAGCTACGAATCGA4190.10627208189291124No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA3950.10018489820453444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAA1253.6379788E-1233.59914816
ATCTGTA1001.2561941E-831.4992037
TAATAGG450.004305595531.1103234
GGATTAA450.004305595531.11032351
TTACCGT450.004305595531.1103234
CGGTGTA2500.030.79921770
ATCCTAG802.4636665E-630.62422430
ACCGGTA1401.2732926E-1129.99923915
ATATAAG1401.2732926E-1129.9992392
AAACGCT500.007205605527.99929216
TATAGAG500.007205605527.9992927
GCAGAGT500.007205605527.99929225
GTACCAC1653.6379788E-1227.5750586
AGAGGTT1800.027.22153365
TCTGTAC657.951191E-426.9223948
AGTTAGA2000.026.24933661
CGTTTCT2400.026.24933617
ATACGTC808.657994E-526.2493345
AATACGC1606.548362E-1126.24933421
TAGAGGT1900.025.7888264
Produced by FastQC (version 0.11.2)