FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245902
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9520.3871460988523884No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC3920.15941309952745403No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC3870.15737976917633853No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC3780.15371977454433067No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC3410.13867312994607608No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAGTA150.002222819370.0000116
GGATAGT200.006945572352.51
TGATTGA200.006945572352.512
TCTGTGT350.001253845240.08
CCTTACA400.002415749235.02
TACGCTA501.7065837E-435.026
CTTAGGC400.002415749235.03
TAACTGC400.002415749235.06
GGTACCG552.9866648E-431.8181845
GACACCT450.004301942431.1111097
AGTTCCG703.479812E-530.032
TCTAGGC604.973041E-429.1666688
GTATGGA604.973041E-429.16666823
ACGCTAT604.973041E-429.16666827
ACTGAAT755.570484E-528.08
CCTACAG500.007199524427.9999985
TGGTTAC500.007199524427.9999986
TTACACT657.9414615E-426.9230774
CATAGGT808.6435015E-526.2516
TACACTG808.6435015E-526.255