FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224589
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9250.4118634483434185No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5450.24266549118612224No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC4540.2021470330247697No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC4330.1927966196029191No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC4030.17943888614313255No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2420.10775238324227812Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC2370.10552609433231369Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCATA200.0069447952.50000449
TAGGATG200.0069447952.5000045
CTAGGAC200.0069447952.50000440
GATACTA305.869246E-446.66666851
TACGAGT305.869246E-446.66666849
ATACGAG350.001253612240.00000448
GATACGA400.002415303635.00000447
TAGCAGT450.00430115431.1111094
ATAAAGG450.00430115431.1111093
TTGGGCA802.456949E-630.6250024
GGTGTAT500.00719821228.00000210
TAGCACC500.00719821228.0000024
AGGTGTA500.00719821228.0000029
CTGTGTG755.5683584E-528.0000029
TAGGGGG906.146616E-627.2222214
CTATATC657.9392974E-426.9230793
TGGGCAC657.9392974E-426.9230795
TTGTGTA2350.026.80851270
GGATTAG808.6402186E-526.2500021
AGCACCC1659.458745E-1125.4545465
Produced by FastQC (version 0.11.2)