FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187774
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT5900.3142075047663681No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA5690.30302384781705666No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4880.2598868852982841No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC4510.2401823468637831No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG4460.2375195713996613No Hit
GACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCAC4330.23059635519294472No Hit
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG4070.21674992277951155No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG3970.211424371851268No Hit
TAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGC3750.19970815980913226No Hit
CTTCCGATCTGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTT3310.17627573572486074No Hit
GTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGA3170.1688199644253198No Hit
TCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGG3010.16029908294013018No Hit
GGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTG2970.15816886256883275No Hit
GATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGA2900.15444097691906228No Hit
CTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAA2880.15337586673341358No Hit
GCAATAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCT2730.14538754034104828No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCG2700.14378987506257523No Hit
GGGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT2600.13846432413433168No Hit
CCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGT2560.1363341037630343No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAA2550.13580154867020994No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2480.13207366302043946No Hit
GCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATAC2430.12941088755631772No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2430.12941088755631772No Hit
GGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAA2350.12515044681372287No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2320.12355278153524982No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2290.12195511625677675No Hit
GGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATG2280.12142256116395242No Hit
CTACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAA2250.11982489588547936No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC2210.11769467551418195No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2190.11662956532853325No Hit
CATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGG2180.11609701023570888No Hit
CCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTG2150.11449934495723582No Hit
ACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAAC2090.11130401440028971No Hit
GCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTA2080.11077145930746535No Hit
GGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATT2080.11077145930746535No Hit
GTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGC2000.10651101856487054No Hit
AGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGC1960.10438079819357311No Hit
GTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACC1910.10171802272945137No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACTC200.00694302652.561
TACGTCT200.00694302652.56
TACCGTC200.00694302652.57
TAAGGAG305.8667746E-446.6666645
TCGATTC404.5800603E-543.7545
TTCAGGG503.4652967E-642.02
GTTTACT350.001253087540.029
TGGGTTA350.001253087540.05
ACCGTTA459.172513E-538.888898
CCGTTAT556.669323E-638.181829
TCTGAGG501.7050489E-435.08
AGTCCCG400.002414298735.012
CATATAA501.7050489E-435.01
GACCGTT501.7050489E-435.07
AGTTGCC400.002414298735.042
TTACAGG400.002414298735.026
GTTATAG400.002414298735.011
TAGGACC652.094716E-532.3076934
ACGGGTA552.9839916E-431.81818423
GTTCAGG552.9839916E-431.8181841
Produced by FastQC (version 0.11.2)