FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207992
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5690.27356821416208316No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3600.17308358013769762No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2840.13654371321973924No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2820.1355821377745298No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2800.13462056232932035No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2580.1240432324320166No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2570.1235624447094119No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG2480.11923535520596945No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2370.11394669025731759No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2170.10433093580522326No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAT150.002222335770.03
CGTATAC305.86824E-446.66666426
ATATCTT305.86824E-446.6666642
TACTATA404.581771E-543.752
ACTATAC350.00125339940.03
GATATCT459.1759204E-538.888891
ATCTGTG852.557499E-937.0588237
TTCCGAT16750.035.1044772
TTAATAC501.7056789E-435.0000043
AACGTCA400.002414894535.028
GTCAAAA400.002414894535.031
GCATAGG400.002414894535.01
GATCTGG4300.034.1860476
ATCTGGG1550.033.8709687
CTTCCGA17400.033.7931061
TCCGATC17400.033.7931063
CCGATCT17100.033.771934
TTCTGGT751.4840662E-632.66666820
CGATCTG9600.032.4479145
TTTTGAC552.98509E-431.8181817