FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266457
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8120.30473960151168855No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3760.14111094848324496No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT2880.10808498181695358No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2770.10395673598366717No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2760.10358144090791385No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACAGT252.3876932E-456.00000415
TTGACTA200.006946206652.517
TACAGTT352.0831205E-550.016
AGTTACA305.8712316E-446.66666419
CCGATCT18550.034.716984
TTCCGAT19500.033.3846132
TCCGATC19550.033.2992323
CTTCCGA19750.033.139241
CGATCTG9900.032.171725
GATCTGG4500.031.111116
TCTAATG450.004302581331.1111092
AATAGTG450.004302581331.1111095
ATCTGGG1900.029.4736827
GATCTAG1352.564775E-1028.5185186
TAAGGTG500.00720058728.0000025
TAGCTCG500.00720058728.00000259
TTAGTAC906.1522624E-627.2222213
CGATCTA4050.026.7901235
CGCTTAA959.362533E-625.78947350
ATCTAAC700.00122408325.02
Produced by FastQC (version 0.11.2)