FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315303
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9350.2965401534397072No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5400.17126383193309294No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5060.16048055362619448No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4220.13383951310326894No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA3740.11861606137588288No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3640.11544450893267745No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC3630.1151273536883569No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT3630.1151273536883569No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA3600.11417588795539528No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT3490.11068718026786932No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA3390.1075156278246639No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA3330.10561269635874064No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG3250.10307545440417631No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC3200.10148967818257358No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA400.00241678135.05
CAGGGGT501.707675E-435.04
CCGTTAA400.00241678135.09
ACTCTCT552.9885658E-431.818188
GAGTGTA1950.030.51282170
CTGAGGT500.0072025627.9999989
TAAACTA500.0072025627.9999985
TTAGTCG500.0072025627.99999870
TATGCTT1554.3655746E-1127.0967735
GGTGTAT959.369645E-625.7894741
GTGTAGG851.3060782E-424.7058831
TATAAGA851.3060782E-424.7058833
AGGGACT851.3060782E-424.70588334
TAGACAG1001.3945806E-524.4999985
CGTGTGA1001.3945806E-524.49999870
AATACTA2001.8189894E-1224.4999985
AAGTAAT1352.3035136E-723.33333465
AGAGTGT2550.023.33333469
AAAGTAA1352.3035136E-723.33333464
ATTCCCT750.00183009623.33333466
Produced by FastQC (version 0.11.2)