FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74539
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4180.5607802626812809No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2260.30319698412911367No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1930.25892485812795984No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC1860.2495338010974121No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1580.211969572975221No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA1240.16635586739827474Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC1100.1475737533371792Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCAA200.00692665552.542
TAATCCT200.00692665552.54
TCAGGGA501.695195E-435.0000043
ATACAGG400.002404978735.06
ACGGCGG604.940186E-429.16666653
TGATAGA657.889257E-426.9230776
GATAGAT851.2930261E-424.7058837
GGTGTAG952.747712E-422.10526570
ATCTGGC952.747712E-422.1052657
GAATTAG6800.021.61764770
GTGTAGA850.00375805420.58823670
ATCTGCG1205.6262525E-520.4166667
GATCTGG2750.020.3636366
TTGGGAG1901.3043973E-820.26315914
CGGTGTA1408.082447E-620.070
GATCTTG1408.082447E-620.06
CGAATTA7850.018.72611469
ATCTGGG1159.960157E-418.2608687
CGATCTT4050.018.1481485
ACGGTGT1551.9444045E-518.06451669
Produced by FastQC (version 0.11.2)