FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147924
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7260.49079256915713476No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2010.1358805873286282No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1840.1243881993456099No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC150.002221275669.99831414
TGCTTTT200.006940789552.49873443
GTGGATC200.006940789552.49873469
TTATTCT200.006940789552.49873415
CCCATAA200.006940789552.49873438
GTGCTTA352.078877E-549.9987911
GCTTATT305.8634084E-446.66554313
TTTCGGA350.001250291940.012561
GTTAGCC350.001250291940.012563
CTGGTAT350.001252373739.99903562
ATCTAGT459.164371E-538.887957
TACTCTT459.164371E-538.8879520
TTAGCCG400.002408931535.010994
GGTCCCC400.00241293334.99915720
AGGGATG400.00241293334.9991577
ATCTGTT552.9813708E-431.8174157
GGTGCTT450.00429695931.1103610
CGTGTGA604.964273E-429.16596470
TAGCCGG500.007179371528.0087935
TCCGTGC500.007191239427.9993258
Produced by FastQC (version 0.11.2)