FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216409
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6810.31468192173153614No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3440.1589582688335513No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2650.12245331756072991No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2360.1090527658276689No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2330.10766650185528327No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAA200.00693874152.5109247
ACTCTCA200.006945086652.4987831
TAGGGGT305.869441E-446.6655854
TACGTGT350.001253654139.99907768
ATACGTG400.002415384634.9991967
TCTATAC708.658171E-734.999193
TCGTTTT450.00430129831.11039214
GCGGTAC450.00430129831.11039237
CGTTCTG450.00430129831.11039210
TCTAGGC604.9717654E-429.165993
AGGTGTA1850.028.37772270
GATACGT500.00719845827.9993566
GAAGAGT500.00719845827.999356
CGGTACG500.00719845827.9993538
TCGATTC1554.3655746E-1127.09614845
TCTGTCC657.939433E-426.92245527
AATCAGG1659.458745E-1125.45395936
GAATCAG1659.458745E-1125.45395935
TCTAGGA1951.8189894E-1225.1276252
TCTAGCT700.00122350424.9994228
Produced by FastQC (version 0.11.2)