FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205181
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8590.41865474873404457No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT4310.21005843620998046No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3100.15108611421135484No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2920.14231337209585682No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2680.13061638260852612No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA2660.1296416334845819No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2570.1252552624268329No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2540.12379313874091656No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2500.1218436404930281No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2470.12038151680711177No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT2300.11209614925358587No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT2230.10868452731978107No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG2140.10429815626203207No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA2060.10039915976625517No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGG207.910522E-570.004633
TAATATC200.006942117652.50347534
GTGTAAC200.006942117652.50347569
CTAGGGT200.006942117652.5034754
ACCGTTA503.4652894E-642.002788
AACTCTA855.638867E-1141.17919565
ATTATAC350.001252950240.00264770
CCGTTTA556.669303E-638.18434527
CCGATCT11900.036.1788644
GACCGTT601.2111381E-535.002327
CTTAATC400.002414032435.00231659
TAAGTCG400.002414032435.00231656
GAATACT400.002414032435.0023164
CGTTATA1102.3646862E-1135.00231651
CGATAGT400.002416922534.99378228
CTTCCGA12650.034.0338751
TCCGATC12650.034.0338753
TTCCGAT12850.033.504162
CGTAAAT751.4831639E-632.6688323
ATTAAGA552.983787E-431.8202881
Produced by FastQC (version 0.11.2)