FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225432
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8230.3650768302636715No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT5170.22933744987401966No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT4070.1805422477731644No Hit
ATCACAAACATTTCAACTTCGCTGAGTCTCGGGAGGAGACAGTGTGGCCA2820.125093154476738No Hit
GTGCTGGAAGATTAATTGATGGGGTGCAAGCTCTTGATCGAAGTCCCAGT2630.11666489229568118No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2440.10823663011462438No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2400.10646225912913873No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2300.1020263316654246No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTC200.00694560952.498540
CTAGGGT305.8701186E-446.665344
ATAACCT451.6800532E-646.66533368
TGCCTAA551.3066347E-744.5441840
TTACTTG556.666425E-638.189237
GTCATAT654.8396214E-737.6995931
TCATATA654.8396214E-737.6995932
CGCCTAC852.561137E-937.05776630
TCTGAAC400.002415661734.9998
GGATTTG904.476533E-934.99914
TAAGACT806.214759E-834.9994
TAACTTA501.7064184E-434.99910
ATATGAC1055.456968E-1033.3323823
TCGCCTA957.588824E-933.1569529
CCTACCC957.588824E-933.1569521
GATTTGC957.588824E-933.1569515
ATATACC751.4851303E-632.6657334
GCCTAAA751.4851303E-632.66573341
CATCCAA751.4851303E-632.66573354
AAGACTA652.0969797E-532.306775