FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114837
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4820.4197253498436915No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT1940.16893509931468081No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC1820.1584855055426387No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1490.12974912266952288No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC1480.12887832318851938No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1350.11755792993547376No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1310.11407473201145973No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA1270.11059153408744568No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA1190.10362513823941762No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG1180.1027543387584141No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1150.10014194031540356No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT150.00221979370.020
TTTGTCA150.00221979370.047
ATGTAGG150.00221979370.01
CCGTAGA150.00221979370.055
AATCCTC200.00693618852.552
TAGCGGA200.00693618852.53
TTACGAT305.857196E-446.66666460
CACCGAG350.001251053640.00000440
ATCTAGC708.609568E-735.0000047
TACCATC450.0042924831.11111312
TAAGTTT450.0042924831.11111331
CAATTCC450.0042924831.11111322
TCTAGCA450.0042924831.1111138
GTGTGAT450.0042924831.11111336
TCAATTC450.0042924831.11111321
TTAAGTT450.0042924831.11111330
TAGGGCA450.0042924831.11111328
GGATTTA450.0042924831.11111366
CGCGTAA450.0042924831.11111321
ACGAGAG450.0042924831.11111350
Produced by FastQC (version 0.11.2)