FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157616
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7870.4993147903766115No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3060.1941427266267384No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2350.14909653842249518No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2160.1370419246776977No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1930.12244949751294285No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA1910.12118059080296417No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1770.1122982438331134No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC1720.10912597705816668No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC1660.10531925692823063No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1630.10341589686326262No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC1600.1015125367982946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA452.683555E-854.4444471
CATATGC200.006940968352.4999963
TTCGCAT200.006940968352.49999639
CCCCTGA305.86389E-446.6666641
TCGGGTA404.576695E-543.74999625
CTGCCTA350.001252475340.028
TCCGACC459.165803E-538.8888935
AGGTAGC601.2099104E-535.07
TCCTAAG601.2099104E-535.02
AGAGTAC400.002413126434.9999963
CGAAATT552.9818312E-431.8181813
TTGTCGG552.9818312E-431.8181822
TATATGC552.9818312E-431.818183
GGTGTAT450.004297297431.11111341
CTGGCCC450.004297297431.1111139
TCTGTCC703.4719644E-530.027
TAAGGTA703.4719644E-530.05
CTTGTCT604.965034E-429.1666669
GCGAAAT604.965034E-429.16666612
TTCCTTG604.965034E-429.16666618
Produced by FastQC (version 0.11.2)