FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354754
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11040.3112015650281603No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC4590.1293854332861645No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA4300.12121075449466391No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4140.11670058688556015No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT4080.11500927403214622No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3600.1014787712048349No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG3580.10091500025369693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACACG503.4740624E-642.00000444
GTGTGCG601.2141814E-535.09
CGATCTG17450.032.4928365
CCGATCT35200.032.0170444
TCCGATC36000.031.7916643
TTTCGGA1550.031.6129061
TTCCGAT36250.031.4758632
TAAACTA450.004304485431.1111135
CACTACA450.004304485431.1111132
CTTCCGA37600.030.5319121
ACTATGC604.977432E-429.1666668
TATGCCG853.948855E-628.8235310
GATCTGG7550.028.7417226
GATCTAG1950.028.7179496
CTGACTA500.00720375528.0000029
CGAGACT500.00720375528.00000220
TACCGGA500.00720375528.0000022
GAGTGTA2900.027.75862170
GTATATG657.9484354E-426.9230771
ATCTGGT1850.026.4864867