FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158691
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8580.5406733841238633No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2100.13233264646388265No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1940.12225015911425348No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT1750.11027720538656888No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC1720.1083867390085134No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1650.10397565079305064No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCCA150.002221837469.9962250
TGGGGAT252.3816607E-456.0146314
CTATGAC200.00693388252.5137143
GTCTCCT200.00693388252.51371415
TAAGGGT200.00693388252.5137145
AGTGGTC200.006942534452.4971668
GTATAGT404.5697747E-543.761431
GAGGACA350.001252833239.99783726
ACATGTT350.001252833239.99783730
TACTAAC459.169E-538.8867877
TCCGTAT400.00241008235.0091442
TATAGTA501.7012424E-435.0091442
TTCGGAA501.7012424E-435.0091442
CCTGTTT400.002413813534.998118
ATGTTGT501.7043999E-434.9981132
CTAACCA751.4817506E-632.66499
AGTACTA552.9773515E-431.8264925
TGTTGTT552.982866E-431.81646233
GTACTAA552.982866E-431.8164626
CCAAACA450.004291891631.1192384