Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n02_adr3wtzt14s6.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 226985 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 917 | 0.4039914531797255 | No Hit |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 876 | 0.38592858558935617 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 789 | 0.34760006167808444 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 784 | 0.3453972729475516 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 608 | 0.26785910963279513 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 416 | 0.1832720223803335 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 408 | 0.17974756041148093 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT | 238 | 0.10485274357336388 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACAGATA | 20 | 7.911354E-5 | 70.006386 | 70 |
AGTGCGT | 20 | 0.006948431 | 52.49323 | 8 |
TTCGGAA | 45 | 9.185554E-5 | 38.88387 | 2 |
TTTCGGA | 40 | 0.0024142757 | 35.003193 | 1 |
ATGGTTG | 80 | 6.22058E-8 | 34.995483 | 14 |
GGTAACG | 85 | 1.0577605E-7 | 32.944183 | 26 |
AACGGGT | 90 | 1.7434104E-7 | 31.120808 | 22 |
ATCCAAC | 45 | 0.0042993263 | 31.11395 | 58 |
GGTACAA | 80 | 2.45535E-6 | 30.627794 | 57 |
GTCCTAG | 95 | 2.8055E-7 | 29.476376 | 1 |
GGTTGCA | 95 | 2.8055E-7 | 29.476376 | 16 |
GAATCAG | 95 | 2.8109207E-7 | 29.469881 | 35 |
TCGATTC | 95 | 2.8109207E-7 | 29.469881 | 45 |
GGGTTCG | 95 | 2.8109207E-7 | 29.469881 | 41 |
TCAGAAC | 60 | 4.969189E-4 | 29.169329 | 61 |
AGGACTA | 110 | 3.165769E-8 | 28.63267 | 5 |
AGACCAT | 100 | 4.3948057E-7 | 28.002558 | 63 |
ATAAGGT | 50 | 0.007195161 | 28.002558 | 69 |
AGGGTTC | 100 | 4.4032822E-7 | 27.996386 | 40 |
CAGGGTT | 100 | 4.4032822E-7 | 27.996386 | 39 |