FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226985
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC9170.4039914531797255No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC8760.38592858558935617No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC7890.34760006167808444No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC7840.3453972729475516No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6080.26785910963279513No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA4160.1832720223803335Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC4080.17974756041148093Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2380.10485274357336388No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGATA207.911354E-570.00638670
AGTGCGT200.00694843152.493238
TTCGGAA459.185554E-538.883872
TTTCGGA400.002414275735.0031931
ATGGTTG806.22058E-834.99548314
GGTAACG851.0577605E-732.94418326
AACGGGT901.7434104E-731.12080822
ATCCAAC450.004299326331.1139558
GGTACAA802.45535E-630.62779457
GTCCTAG952.8055E-729.4763761
GGTTGCA952.8055E-729.47637616
GAATCAG952.8109207E-729.46988135
TCGATTC952.8109207E-729.46988145
GGGTTCG952.8109207E-729.46988141
TCAGAAC604.969189E-429.16932961
AGGACTA1103.165769E-828.632675
AGACCAT1004.3948057E-728.00255863
ATAAGGT500.00719516128.00255869
AGGGTTC1004.4032822E-727.99638640
CAGGGTT1004.4032822E-727.99638639