FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146673
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5180.3531665678072992No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC3270.2229449182876194No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2990.2038548335412789No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2910.19840052361375307No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC2770.1888554812405828No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT1810.12340376211027251No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG1540.10499546610487273No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC150.002220766670.00204559
TGGGTAT150.002220766670.0020456
ACCGGTA200.006939203552.50153415
TATAGGT200.006939203552.5015343
TTGATGA200.006948571752.48363566
TATGGTG501.7029353E-435.0010265
TAGGACC501.7029353E-435.0010264
ATCAGAC400.002412232335.0010227
CTATGGG552.9803108E-431.8191132
GTAGCCT450.004295714731.11201923
TAACCCT450.004295714731.1120194
ACCGTTA450.004295714731.1120198
TTAGGAC703.469633E-530.0008773
TGAACAC500.007189160728.000823
TAAGTTA500.007189160728.0008254
CGTTACC657.924726E-426.92386258
ACCCGTT657.924726E-426.92386262
CTTAGGA808.618429E-526.2507672
TTGTGTA1101.00955E-625.44661170
GTATTTA700.001221247425.0007321