FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289298
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18030.6232327911012174No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4320.1493269915450504No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC3750.1296241246050785No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC3170.10957559333282635No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC3090.10681027867458469Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2910.10058832069354091Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2900.10024265636126072No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTAT200.006943479652.5063551
TTTACCG350.001254531239.9979331
TCTAGGG708.661809E-735.004242
TCTGTGG552.9888673E-431.8165348
AAGTCAC450.00430063631.11487868
CCTGATT853.9471342E-628.82203548
GTGTAGA1950.028.72142470
GTATCGA500.007197336828.0033871
CTCTAGG500.007197336828.0033871
ATAGCGT700.001224706124.9987056
AGTTCCG851.3061735E-424.70460132
ATCTGTG2200.023.86247
ATTCAAG750.00182844323.3361591
GTATAGT901.9224435E-423.3361591
TAAGTTC750.001830283623.33212530
TGTATGG750.001830283623.3321255
GTACCGT901.9246979E-423.3321236
ACAGGTC952.775415E-422.1041163
ATACCGC952.775415E-422.1041166
TGCATTA800.002663172321.87386712