Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n02_adr3wtzt14s68.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 289298 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 1803 | 0.6232327911012174 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 432 | 0.1493269915450504 | No Hit |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 375 | 0.1296241246050785 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 317 | 0.10957559333282635 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 309 | 0.10681027867458469 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 291 | 0.10058832069354091 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT | 290 | 0.10024265636126072 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCTCTAT | 20 | 0.0069434796 | 52.506355 | 1 |
TTTACCG | 35 | 0.0012545312 | 39.99793 | 31 |
TCTAGGG | 70 | 8.661809E-7 | 35.00424 | 2 |
TCTGTGG | 55 | 2.9888673E-4 | 31.816534 | 8 |
AAGTCAC | 45 | 0.004300636 | 31.114878 | 68 |
CCTGATT | 85 | 3.9471342E-6 | 28.822035 | 48 |
GTGTAGA | 195 | 0.0 | 28.721424 | 70 |
GTATCGA | 50 | 0.0071973368 | 28.003387 | 1 |
CTCTAGG | 50 | 0.0071973368 | 28.003387 | 1 |
ATAGCGT | 70 | 0.0012247061 | 24.998705 | 6 |
AGTTCCG | 85 | 1.3061735E-4 | 24.704601 | 32 |
ATCTGTG | 220 | 0.0 | 23.8624 | 7 |
ATTCAAG | 75 | 0.001828443 | 23.336159 | 1 |
GTATAGT | 90 | 1.9224435E-4 | 23.336159 | 1 |
TAAGTTC | 75 | 0.0018302836 | 23.332125 | 30 |
TGTATGG | 75 | 0.0018302836 | 23.332125 | 5 |
GTACCGT | 90 | 1.9246979E-4 | 23.332123 | 6 |
ACAGGTC | 95 | 2.775415E-4 | 22.104116 | 3 |
ATACCGC | 95 | 2.775415E-4 | 22.104116 | 6 |
TGCATTA | 80 | 0.0026631723 | 21.873867 | 12 |