FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234777
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6850.29176622923029083No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC3290.14013297725075285No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3000.12778083031983542No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT2960.12607708591557096No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA2630.11202119458038906No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG400.00241552735.01
GAAGTAC400.00241552735.06
ATATAGG450.004301548531.1111092
TTATCCG500.0071988727.99999810
TGTAAGG500.0071988727.9999982
ACGACTA500.0071988727.99999863
TATATGT657.94038E-426.9230774
GTGTAAG657.94038E-426.9230771
ATCTAGC808.641863E-526.257
AGGTTCG808.641863E-526.2567
TATTACT959.356334E-625.7894735
AGTAATA700.001223648425.066
TCTATGC700.001223648425.08
TAGAGGT851.3046895E-424.70588364
AGAGGTT851.3046895E-424.70588365
ATTAGAG1001.3926068E-524.49999820
GGGCTGG1001.3926068E-524.49999811
TGCTTAA1001.3926068E-524.49999827
CATTAAT1001.3926068E-524.49999855
CGTGTGA1001.3926068E-524.49999870
Produced by FastQC (version 0.11.2)