FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94145
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4750.5045408678102926No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT1180.12533857347708321No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG1070.11365446916989749No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA1040.11046789526793777No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC950.10090817356205853No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC950.10090817356205853No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTCG200.00693231852.528
ACCTGAG200.00693231852.57
AATTGGC200.00693231852.555
TAGTGTA604.8585207E-946.66666470
GCCGGGA350.001249903240.00000413
CAATAAG350.001249903240.0000041
GTACTAT556.6339144E-638.1818161
CCGGGAT400.002408201335.014
TGACAAG400.002408201335.02
CGGGATC400.002408201335.015
AGAGGTT400.002408201335.065
TAAAGCA450.004288581731.11110931
AGGACTA450.004288581731.1111095
TACTATT450.004288581731.1111092
ATAGTGT1103.1204763E-828.63636270
ACCGGGC500.00717730127.9999988
TACCATG500.00717730127.99999810
ATAGGGG500.00717730127.9999983
ATTACTT500.00717730127.9999986
AGGTTCG500.00717730127.99999867