FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91711
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2660.29004154354439493No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA1960.2137148215590278No Hit
CGTTGAGACAGTGCCCAGATCGTTACGCCTTTCGTGCGGGTCGGAACTTA1710.1864552779928253No Hit
ATCCTAAGGTGAGCGAGTGAACTCTCGTTAAGGAACTCGGCAAAATGACC1090.11885160994864304No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA940.10249588380892151No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGA150.002218363370.01
GTATTAA150.002218363370.01
TCCCATC150.002218363370.042
ACGAACA150.002218363370.048
AACGTTT200.006931756652.556
CATATAT200.006931756652.53
GGTCGAA200.006931756652.512
GAATATA200.006931756652.51
ATATCTA200.006931756652.56
GAACGTT200.006931756652.555
CTTCTTG200.006931756652.59
CGCAACA200.006931756652.567
TCAACTA200.006931756652.547
ATCTGTA200.006931756652.57
CAGGGTG305.85099E-446.6666644
TGTACTG305.85099E-446.6666645
GAGGACT350.001249736440.027
AGCATGA350.001249736440.029
GTACTGT350.001249736440.06
GTGAGGA350.001249736440.025
Produced by FastQC (version 0.11.2)