FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163871
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5790.3533267021010429No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC1720.10496060925972259No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCCA305.8645743E-446.66666424
TCAATCA305.8645743E-446.66666413
TCTGCGG459.1673995E-538.8888858
TCTGACC501.7041022E-435.0000048
TTAATGC400.00241340435.0000043
ACTCGGT400.00241340435.00000424
AGGCTGG400.00241340435.0000046
GGCAAGT652.093097E-532.30769320
GAATACA450.004297791531.1111091
TCCTACC450.004297791531.1111094
CTACCGT450.004297791531.1111094
ATGCGTT450.004297791531.1111096
GATCTTG1401.2732926E-1130.06
GAGTAGG604.965883E-429.16666640
ACCGTGA604.965883E-429.1666668
ATGGGTT500.0071926228.0000025
CTCCGAT500.0071926228.00000233
TCCGATG500.0071926228.00000234
AATGCGT500.0071926228.0000025
ACAGGGC500.0071926228.0000028