FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104836
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3470.3309931702850166No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2630.25086802243504136No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG1990.1898202907398222No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC1780.1697890037773284No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA1780.1697890037773284No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1500.14308062116067No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1480.1411728795451944No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1380.1316341714678164No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC1360.1297264298523408No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA1240.11827998015948721No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA1190.1135106261207982No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1170.1116028845053226No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC1130.1077874012743714No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA1080.10301804723568239No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAA200.00693450852.558
CTGAGGG200.00693450852.512
TCTACTG200.00693450852.58
CGTAATC200.00693450852.53
ACGTAAT200.00693450852.52
ATACCAT200.00693450852.56
GTTAGAG305.854845E-446.66666462
TAGAGGT350.001250554840.064
TCATTAA350.001250554840.043
CATTAAT350.001250554840.044
AGTTAGA350.001250554840.061
TGTACTG501.6999182E-435.0000045
AAGGCTC501.6999182E-435.00000470
TTAGAGG400.002409448135.063
GGACGGG400.002409448135.06
AGTATCT751.4747711E-632.66666855
TAGTATC751.4747711E-632.66666854
ATCTATG552.97506E-431.818187
AAGATGT552.97506E-431.8181834
AAAGGCT552.97506E-431.8181869
Produced by FastQC (version 0.11.2)