FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190720
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5260.2757969798657718No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3700.194001677852349No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2730.14314177852348994No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2520.13213087248322147No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2420.12688758389261745No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2370.12426593959731544No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2370.12426593959731544No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2120.11115771812080537No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1980.10381711409395973No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC1980.10381711409395973No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1960.10276845637583892No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACA200.00694319352.561
CGACCCG305.8670086E-446.66666437
TCTGACC753.5157427E-837.3333328
TTGTGTA1400.035.00000470
ACAGTAC501.7051492E-435.08
GTACAAA400.002414393535.01
TGTCTAA703.4757548E-530.00000234
TTGTCTC604.968904E-429.16666610
AGAATAT604.968904E-429.16666617
GTTTATC500.007195533727.99999843
ACAAAGG657.9348835E-426.9230773
TTCTTAT657.9348835E-426.92307741
CTGTGAA657.9348835E-426.9230779
GCATAGG657.9348835E-426.9230771
TACAAAG959.3443105E-625.7894732
CTTGTCT700.001222805525.0000029
TGGAGTT700.001222805525.00000236
TATGCTT700.001222805525.0000025
AACGAAA700.001222805525.00000256
TACTATA700.001222805525.0000022
Produced by FastQC (version 0.11.2)