FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273704
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13430.4906760588080554No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4950.18085230760237336No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC4330.15820009937742963No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC3750.13700932394119195No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC3380.123491070645661No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2900.10595387718118844Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCAG305.873174E-446.6639968
TAGTTCA404.5871457E-543.7474865
CCTCACA459.186643E-538.8866542
AAACACG708.67165E-734.99799315
GTGTACC400.002416905734.9979918
TGGGACT1103.1628588E-828.6399534
GTATTTG500.007202944327.9983921
TGGACGT1004.4061198E-727.99839226
ACTCTGC1154.8785296E-827.3897348
ACACAGC1056.748396E-726.6651341
CTGCAAA1056.748396E-726.66513411
ACGAATG700.001223273525.00313445
ATCTAGT851.3059721E-424.7044647
GCACTCT1151.4916222E-624.346436
AACACGA1151.4916222E-624.3464316
TGGCGTA901.9220203E-423.33625650
AGTTCCG901.9220203E-423.33625632
AGCACTC1202.15999E-623.3319955
CGTATAG1052.0341815E-523.33199330
AGTGGAC1052.0341815E-523.33199324