Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n02_adr3wtzt14s60.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 273704 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 56 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 1343 | 0.4906760588080554 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 495 | 0.18085230760237336 | No Hit |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 433 | 0.15820009937742963 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 375 | 0.13700932394119195 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 338 | 0.123491070645661 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 290 | 0.10595387718118844 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATATCAG | 30 | 5.873174E-4 | 46.66399 | 68 |
TAGTTCA | 40 | 4.5871457E-5 | 43.747486 | 5 |
CCTCACA | 45 | 9.186643E-5 | 38.886654 | 2 |
AAACACG | 70 | 8.67165E-7 | 34.997993 | 15 |
GTGTACC | 40 | 0.0024169057 | 34.99799 | 18 |
TGGGACT | 110 | 3.1628588E-8 | 28.63995 | 34 |
GTATTTG | 50 | 0.0072029443 | 27.998392 | 1 |
TGGACGT | 100 | 4.4061198E-7 | 27.998392 | 26 |
ACTCTGC | 115 | 4.8785296E-8 | 27.389734 | 8 |
ACACAGC | 105 | 6.748396E-7 | 26.665134 | 1 |
CTGCAAA | 105 | 6.748396E-7 | 26.665134 | 11 |
ACGAATG | 70 | 0.0012232735 | 25.003134 | 45 |
ATCTAGT | 85 | 1.3059721E-4 | 24.704464 | 7 |
GCACTCT | 115 | 1.4916222E-6 | 24.34643 | 6 |
AACACGA | 115 | 1.4916222E-6 | 24.34643 | 16 |
TGGCGTA | 90 | 1.9220203E-4 | 23.336256 | 50 |
AGTTCCG | 90 | 1.9220203E-4 | 23.336256 | 32 |
AGCACTC | 120 | 2.15999E-6 | 23.331995 | 5 |
CGTATAG | 105 | 2.0341815E-5 | 23.331993 | 30 |
AGTGGAC | 105 | 2.0341815E-5 | 23.331993 | 24 |