FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674320
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC35320.523786926088504No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC31640.4692134298256021No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC29110.43169415114485704No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC27480.4075216514414521No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA14690.21784909241902956Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC14540.21562462925613954Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14440.21414165381421282No Hit
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC8360.12397674694507059No Hit
GTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACG7070.10484636374421638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAT451.6847607E-646.66661569
AACGTAT400.002418739534.9999619
TTATAGC450.004307233331.1110764
TATAGCC450.004307233331.1110765
AGAGTGA952.8217073E-729.47365470
AATATGT500.007208327827.999976
TAAGCGG500.007208327827.9999767
TATAGTA2250.026.4444182
CGATATA1207.4003765E-826.24997163
TACTAAC2350.025.3191227
ATTAGCC700.001226036324.9999733
GAACGTA700.001226036324.99997318
GTTTTAT851.3075868E-424.707691
GTCCTAG1851.4551915E-1124.5963921
AGTCACC1301.6056038E-724.23074369
AATTGTA2650.023.7735617
GTATAGT2550.023.335041
TATGGTG1052.039024E-523.333315
TAACCAC2600.022.8845910
AAGTCAC1403.282803E-722.49997768
Produced by FastQC (version 0.11.2)