H3W35AFXY_n02_adr3wtzt14s59.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3W35AFXY_n02_adr3wtzt14s59.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172595
Sequences flagged as poor quality	0
Sequence length	76
%GC	55

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	695	0.40267678669718127	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	331	0.19177844085865756	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC	280	0.1622294967988644	No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	247	0.1431095918189982	No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC	243	0.14079202757901446	No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	207	0.11993394941916045	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA	192	0.11124308351922128	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGAGTG	15	0.0022216926	70.0	9
TAGGGCA	15	0.0022216926	70.0	4
AGCTCCG	20	0.006942079	52.5	5
TGGTACA	20	0.006942079	52.5	19
ACGATAT	30	5.8654463E-4	46.666664	62
TACGATC	30	5.8654463E-4	46.666664	61
GTTTACT	50	3.4636596E-6	42.0	29
TTACGAT	60	2.5827103E-7	40.833332	60
CTTACGA	55	6.666185E-6	38.181816	59
ATTACCG	40	0.002413759	35.0	5
TACGATA	40	0.002413759	35.0	61
AGATTGC	40	0.002413759	35.0	20
TTTACTG	65	2.0937381E-5	32.307693	30
AACGTCT	45	0.0042984188	31.111109	67
AAACGTC	45	0.0042984188	31.111109	66
AAGTTCC	45	0.0042984188	31.111109	31
ATCTTAG	45	0.0042984188	31.111109	7
TTAGGAC	100	4.388021E-7	27.999998	3
GCGGTCT	50	0.0071936646	27.999998	9
CATAGGG	50	0.0071936646	27.999998	2