FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93821
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT2380.2536745504737746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1870.19931571822939426No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG150.002218522470.01
TAACGTT150.002218522470.031
TCTGCCA200.00693224952.526
TATCATT200.00693224952.518
AGGAAAG200.00693224952.514
GTATCAT200.00693224952.517
GTCCCAT200.00693224952.51
TAGGACG200.00693224952.54
CTTAAGG451.6691683E-646.6666643
ATCTAAC305.85168E-446.6666647
TAGGAGA305.85168E-446.6666644
GATCTAA703.765308E-1045.0000046
TGCTAAT404.5624533E-543.752
TCGACCG350.001249882940.00000418
TCTGGCC350.001249882940.0000048
ATCTGTG459.137429E-538.8888857
AGTATCA400.00240816235.016
GCCTGAT400.00240816235.021
TACTGGT400.00240816235.02
GTATTTA652.083618E-532.3076931
Produced by FastQC (version 0.11.2)