FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99535
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3520.35364444667704825No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2570.2582006329431858No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2280.22906515296126992No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA1850.1858642688501532No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA1660.16677550610338074No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1510.1517054302506656No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC1510.1517054302506656No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC1480.14869141508012257No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA1400.14065404129200784No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1370.1376400261214648No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG1220.12256995026874969No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC1210.12156527854523533No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1170.11754659165117798No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC1150.11553724820414929No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT1110.11151856131009193No Hit
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAA1040.10448585924549153No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGA1000.10046717235143417No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA1000.10046717235143417No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT1000.10046717235143417No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAATT150.00221955269.9949811
CAGAATC200.006935451652.49622726
TCCATTA200.006935451652.4962272
AATAGGG200.006935451652.4962272
TGGAGTT602.56945E-740.83040236
TACTATA602.56945E-740.8304022
ATACCTT602.56945E-740.8304026
CCAGAAT350.001247600840.0172425
GAATCCC350.001250691139.99712828
AGAATCC350.001250691139.99712827
CCTTTAT556.641032E-638.179089
GGACTAC852.5320332E-937.0561646
AGGACTA852.5320332E-937.0561645
CTAGGAC852.5320332E-937.0561643
ACTATAC601.2060285E-534.997493
TTCTTAT601.2060285E-534.9974941
CTTTATG601.2060285E-534.9974910
TATTCCG400.002409712934.99748658
GGAGTTC708.599036E-734.99748637
TAGGACT904.425601E-934.9974864
Produced by FastQC (version 0.11.2)