FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279861
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17670.6313848660585075No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT3070.10969731402374751No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAAT200.00695056752.4923671
ACAACAG556.67954E-638.1830835
ATCTGGT1300.037.6935587
GCTAGGA400.002415923235.00116335
TTTCGGA2250.034.2172431
TACAGGC552.9871296E-431.8192372
CGATCTG14550.031.7536325
TTCCGAT28900.030.0355972
ATCTAGC703.4807206E-530.0009967
CCGATCT28050.029.9475174
TCCGATC29400.029.2866863
ATAGGAC604.973808E-429.1676353
CTTACAC604.973808E-429.1676353
CTTCCGA29900.028.5577181
ATAAGCA755.5719305E-528.0009336
CTTAAAC500.00720002928.0009273
CAATAGG500.007206314227.9959261
GATCTGA3650.027.8091416
CTGTAAG906.160677E-627.2182621
CCTATAA657.9509703E-426.919161
Produced by FastQC (version 0.11.2)