FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191796
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT9770.5093953992784No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT6450.3362948132390665No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6440.3357734259317191No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA6260.32638845439946607No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG6240.32534567978477136No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA6020.31387515902312874No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC5710.29771215249535965No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4780.2492231329120524No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT4750.24765897099001022No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT4740.24713758368266284No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA4700.24505203445327325No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG4660.2429664852238837No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4570.23827399945775718No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA4550.23723122484306242No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA4350.22680347869611464No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4160.21689711985651422No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA4120.21481157062712466No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT3970.2069907610169138No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGA3950.205947986402219No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA3930.20490521178752424No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA3920.20438382448017683No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG3880.20229827525078728No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA3800.1981271767920082No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAA3450.17987862103484953No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC3370.17570752257607042No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC3240.16892948758055434No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGT3190.16632255104381738No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA3150.16423700181442785No Hit
GTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTT3090.1611086779703435No Hit
AGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAG3040.15850174143360654No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC3000.15641619220421699No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAA2960.15433064297482743No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC2920.15224509374543785No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACT2880.1501595445160483No Hit
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTA2870.1496381572087009No Hit
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAA2810.14650983336461657No Hit
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAA2730.14233873490583746No Hit
GGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA2720.14181734759849005No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA2690.14025318567644787No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGG2690.14025318567644787No Hit
CTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCATAT2690.14025318567644787No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTA2650.13816763644705835No Hit
AGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGT2580.13451792529562662No Hit
ATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTT2540.13243237606623703No Hit
ACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAATCCTTTA2490.1298254395295001No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2430.12669711568541575No Hit
CGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAAC2360.12304740453398402No Hit
ATCAGATACCGCCCTAGTTCTAACCATAAACGATGCCAGCTAGCAATTGG2320.12096185530459447No Hit
TTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATG2300.11991908068989968No Hit
GTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATTCTTGGACCGTC2300.11991908068989968No Hit
GTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATG2230.11626936953846795No Hit
GTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCT2190.11418382030907841No Hit
GGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTT2150.11209827107968884No Hit
CTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCATGGTAGTC2120.11053410915764667No Hit
CTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAG2050.10688439800621494No Hit
GTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACT2040.10636301069886754No Hit
TTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACTGT2040.10636301069886754No Hit
CTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGA2030.10584162339152016No Hit
ATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTC2000.10427746146947799No Hit
CCATAAAAGTAGCTACACCCAATTGCTAGCTGGCATCGTTTATGGTTAGA1990.10375607416213059No Hit
CATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAAATGTCTGC1990.10375607416213059No Hit
CCCTAGTTCTAACCATAAACGATGCCAGCTAGCAATTGGGTGTAGCTACT1980.10323468685478321No Hit
CCTCTAACTTTCGTTCTTGATTAATGAAAACATCTTTGGCAAATGCTTTC1920.10010636301069886No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACTT200.006943250552.549
TCTTACC200.006943250552.52
CTTGGGG200.006943250552.53
TAGGGAT200.006943250552.514
ATGAATA200.006943250552.511
ATTATCT200.006943250552.510
GTGTAGA703.8198777E-1045.070
TTCGGAA459.173249E-538.8888852
TTTGTAG400.002414426535.03
TGGAACT400.002414426535.066
CTATTCC450.004299601531.1111094
TGTGTAG1052.0079824E-830.00000269
AGGATTA703.4758537E-530.05
TTCAGGA604.9690035E-429.1666682
TCTATTC604.9690035E-429.1666683
GCACCCC500.0071956328.00000211
CTCTCGA500.0071956328.0000021
TCAGGAT755.564177E-528.03
CTGAACC755.564177E-528.030
AATCAAT1154.859976E-827.3913046
Produced by FastQC (version 0.11.2)