FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150105
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5850.3897271909663236No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2440.16255287965091103No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2050.1365710669198228No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC1760.11725125745311615No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1660.11058925418873455No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1580.10525965157722927No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1530.10192864994503847No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA1520.1012624496186003No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG252.3810707E-456.0154575
ACTTATG200.006941895552.49699810
GTCTAGG350.001250584340.0110471
AATTAAG350.00125263639.99771550
TACATCA400.00240949435.0096632
GGGAAAT400.00240949435.00966316
GGGGGTC400.002413437534.99799711
TGTACCG1055.4205884E-1033.3425375
GTCTTGT957.530616E-933.167051
TACCGAC957.554263E-933.1567
CATCACC552.9763312E-431.8269654
TTGTACC1001.2416422E-831.5086964
GTATCTA1001.2456439E-831.49820156
ACTTTTG1001.2456439E-831.49820143
GTAGTAT1001.2456439E-831.49820153
ATCACCT450.004290848531.1197015
GTCTTAC1151.4351826E-930.4431881
GTACCGA1052.0028892E-829.998296
AGGACTA1052.0028892E-829.9982962
AATGTCT1052.0028892E-829.9982927