FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99665
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6720.6742587668690112No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGC252.3804192E-455.99999641
TAGAGGT200.006933513652.54
TCCTATT200.006933513652.516
TGGTTAT200.006933513652.513
ATTGGAT200.006933513652.556
CGTCAAT200.006933513652.523
GTAGTAG200.006933513652.51
TTGGGGA200.006933513652.513
GCTACAA305.853449E-446.66666819
ATTGCGA503.4488803E-642.018
TCTAGCC350.001250258640.08
TGCGATG350.001250258640.020
CTAGGCC459.141541E-538.88888542
ATCTAGC501.6993198E-435.07
TTTCGGA806.153823E-835.01
AAGTTTA400.002408881735.068
TAGGCCA400.002408881735.043
GTCCAAG400.002408881735.064
TTCGGAA851.0484837E-732.9411772
ATCTGCA751.4738052E-632.6666687
Produced by FastQC (version 0.11.2)