FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517983
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC27560.5320637935994039No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC25800.49808584451613275No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC24740.47762185245461725No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC22670.4376591509759973No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA11660.22510391267667085Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC11510.22220806474343752Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10560.20386769449962644No Hit
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC6740.13012010046661765No Hit
GTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACG5750.11100750410727765No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATC200.00694795952.5036933
TACATAA200.00694795952.5036935
TACGAGT200.00695061252.49862349
ATGGTAT200.00695061252.49862362
AGTCTAC305.877156E-446.6654469
ATTGAGC400.002418521334.99908447
ACTATTG601.2154771E-534.9990860
ATACAAG501.709452E-434.9990858
GACTATT552.991662E-431.81734859
GCCCCCA450.00430684931.11029857
AAGTCTA450.00430684931.11029868
TTACGAG703.4878038E-529.99921468
AGGGCTC604.9785076E-429.1687165
TCCGGAA500.00720429528.001972
GTTCTAT500.007207691727.99926644
CGAGATA755.5832264E-527.99926670
TGCAACC500.007207691727.99926668
ACAGATA1154.8949005E-827.39058566
AGACTAT657.9546164E-426.92237358
TTTCGGA808.657507E-526.2518461
Produced by FastQC (version 0.11.2)