FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193089
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8790.45523048956698725No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT2390.12377711832367458No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT2390.12377711832367458No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA2260.1170444717202948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT200.006939357652.507589
AGTAACA200.006946472552.493982
ACCTAGG305.8705057E-446.6613161
ATCTAGT404.5766392E-543.7563177
ATATTAA404.583646E-543.7449841
ATCTAGC855.638867E-1141.182417
AAGAGTG350.001252285740.005777
GATCTAG2150.039.0754056
CGATCTG11700.038.2961245
GATCTGA3250.037.6977466
CCGATCT23800.036.1816944
TTCCGAT23950.035.7996522
TCCGATC24700.035.2793543
TCTGACC601.2101214E-535.0050548
TCTGGAC806.197297E-835.005058
TATTAAC501.7063766E-434.9959872
CTTCCGA25050.034.3673171
CGATCTA4700.034.260265
GTCAGGG552.9817736E-431.82277712
ATCTGAT552.9817736E-431.8227777
Produced by FastQC (version 0.11.2)