FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152878
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5880.3846204162796478No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACC252.3844613E-456.0000044
TACCCCC200.006940569352.55
CCTAGTG200.006940569352.52
CCATAAC350.001252356940.03
ATCTAGG501.7035675E-435.0000047
AAGACCG400.002412899235.05
TACTATA400.002412899235.02
ACTATAC450.004296896531.1111133
CTACTCG450.004296896531.11111359
ATTACTT450.004296896531.1111136
CTATACC450.004296896531.1111134
ACAATAC450.004296896531.11111368
TTAATAG500.00719113228.0000023
CTGTACT500.00719113228.0000024
ATCTAGC500.00719113228.0000027
CTGCGTA500.00719113228.00000221
ATCCATG500.00719113228.0000021
GATCTAG1653.6379788E-1227.5757586
ATCCTTT657.927635E-426.9230777
TGCCCCC657.927635E-426.92307747
Produced by FastQC (version 0.11.2)