FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154788
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5000.32302245652117734No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAAC200.006940731352.52
GTATTAG200.006940731352.528
TATGAGG200.006940731352.52
CTTGTCT604.8930815E-946.6666689
AGTGTAA305.863558E-446.6666688
AGATTAA602.5804002E-740.83333619
GATTAAG602.5804002E-740.83333620
ATATGCT801.4078978E-939.3754
GGGTGCG459.165038E-538.8888859
TTGTCTC654.833855E-737.69230710
GTTATAT753.508285E-837.3333361
TTTGTAC400.002412991635.061
ATGCTTG806.191658E-835.06
TCCAAAG400.002412991635.049
AAGATTA708.6353975E-735.018
CCGTTTA501.7036658E-435.027
AAAGATT708.6353975E-735.017
TATGCTT904.456524E-934.9999965
CTCAAAG751.4808138E-632.66666814
TAAGCCA751.4808138E-632.66666823
Produced by FastQC (version 0.11.2)