FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143483
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5810.4049260191102778No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATTA305.862118E-446.66666424
GAGCCAT305.862118E-446.66666422
CTATACA350.001252099240.04
TACGGAT350.001252099240.03
CATTACC350.001252099240.026
GTGAGCC350.001252099240.020
ATCCGAC400.002412405335.047
TTACCCC400.002412405335.028
TGAGCCA400.002412405335.021
CCATTAC400.002412405335.025
TAATCCG400.002412405335.045
TTCGGAA851.0539952E-732.9411772
TTTCGGA751.4798134E-632.6666681
AGTGTAG652.0912888E-532.30769370
GGATCGT552.980504E-431.8181846
CATACAG552.980504E-431.8181845
TAGTGTA1001.24418875E-831.49999870
CTGTACT450.004296024331.1111094
TTTACAA450.004296024331.11110938
ACTAGCT450.004296024331.11110939
Produced by FastQC (version 0.11.2)