FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193462
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5990.3096215277418821No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAAGG350.001252021140.0075343
TCTTAGG400.002412248435.006592
GACACTA400.002415310634.99754329
ATCTAGC652.0923439E-532.3137787
TTAAGAC450.00429573231.116972
GGTGATT450.00429573231.1169711
ACTCCCA450.00429573231.1169720
CTGGGTA450.00429573231.116979
ATCTGTG703.4715413E-530.0056517
ACAGTGT604.9636845E-429.1721618
TAAGACT500.007189168628.0052743
ATCTGTT755.5572855E-528.0052747
TACACAG657.9265685E-426.9281485
GATCTGG5450.024.4019576
CGTTCTG750.001825548423.33772910
TCAGGGG750.001825548423.3377293
CGATCTG14450.023.2569755
ATCTGGG2450.022.8614487
AGGTGTA1905.5479177E-1022.10371470
GTTGGCG800.00266029721.87346537
Produced by FastQC (version 0.11.2)