FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258096
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13080.5067881718430352No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5640.2185233401525014No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT4060.15730580869133967No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA3800.14723203769140164No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA3430.13289628665302833No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT3380.1309590229991941No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG3370.13057157026842725No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC3100.1201103465377224No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT2970.1150734610377534No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG2930.11352365011468601No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA2870.11119893373008492No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2680.10383733184551486No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT2610.10112516273014692No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTC150.00222306669.9990345
TCCGTAC308.362451E-658.33252723
CCTTAAT200.00694101852.509451
ACCACTA200.006946340752.499278
GATTAGT200.006946340752.4992744
ATTAGAA305.8656774E-446.6750683
TATTAGA305.8656774E-446.6750682
CCGTACA350.001254045939.99944724
AGTATTC400.00241613434.99951642
GATCTAG1700.032.940726
ATCTAGC652.09794E-532.3072477
TTCCGAT20600.032.287362
CCGATCT20750.031.5478464
AGGACTA2350.031.2822255
CTTGTCT1357.2759576E-1231.1106839
TCCGATC21350.030.8252163
GAGTGTA2050.030.73128370
TAGGACT2400.030.6305144
TATGCTT1500.030.338795
CTTCCGA21600.030.306381
Produced by FastQC (version 0.11.2)