FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604605
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC18050.2985420233044715No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16750.2770403817368365No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC15250.25223079531264214No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC15020.248426658727599No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC13550.22411326403188858No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC8700.143895601260327Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA8610.14240702607487532Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT7990.13215239701954168No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGAGT308.376128E-658.3323674
TCGTATA404.592658E-543.7492863
TTTCGGA1250.039.1993521
ACGATAG1850.034.05349369
CGTGTAG4700.028.29740569
ACACGAT755.5843833E-527.99953767
TTAAGGT755.5843833E-527.9995374
TCACTAT1004.4194348E-727.9995379
ATGGTAT500.0072082927.9995376
CACGATA657.9557195E-426.92263268
TTACACT808.6649765E-526.2495674
GTACTAG1358.072675E-925.9254971
TTCACTA1101.0157164E-625.4541248
GTTCACT1101.0157164E-625.4541247
GACGATA1659.640644E-1125.45412468
CGATAGA1951.8189894E-1225.12778970
TAGGCTG700.001226000324.9995865
TACACTG1401.1888915E-824.9995865
ACTAGTT851.3081648E-424.7054733
TACTAGT851.3081648E-424.7054732
Produced by FastQC (version 0.11.2)