FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67966
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5770.8489538887090604No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACTA150.002215882370.05
AAACTGC200.006924060652.50000420
AATCAGG200.006924060652.50000436
ATCAGGG200.006924060652.50000437
CTGAGAA200.006924060652.50000464
TCGATTC200.006924060652.50000445
AATGCTC200.006924060652.5000045
ATTCCGG200.006924060652.50000448
AACGGGT200.006924060652.50000422
AGAAACG200.006924060652.50000467
GCCTGAG200.006924060652.50000462
ACAGGAA200.006924060652.5000043
TACTAAT200.006924060652.5000047
TAGGACT200.006924060652.5000044
TACACCG200.006924060652.50000439
AGTCGGT200.006924060652.5000046
ATCTCTC305.8402226E-446.6666647
TTTCGGA305.8402226E-446.6666641
GATCTCT556.606697E-638.1818166
GATCTGG1250.036.3999986
Produced by FastQC (version 0.11.2)