FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198492
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7170.3612236261411039No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2410.12141547266388569No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2150.1083167079781553No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC2030.10227112427704894Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2000.10075972835177237Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG200.006943606352.51
TATAGAG350.001253260140.0000045
AGAGACC350.001253260140.0000048
TGTAGGG459.1744056E-538.888892
GTAGGGG601.2114911E-535.03
CTGTAGG552.9846013E-431.818181
TAAAGAA552.9846013E-431.818185
TCAGTTA450.00429995931.1111138
TAGGACT802.4550482E-630.6254
AGGACTA703.47646E-530.0000025
CTAGGAC703.47646E-530.0000023
TTTCGGA952.804736E-729.4736861
TTCGGAA952.804736E-729.4736862
TCTGTGG853.9373335E-628.823538
CATATGT500.00719622927.9999984
GAGTAGG500.00719622927.9999981
AAGACTC500.00719622927.9999984
TAGCAGA500.00719622927.9999984
GACATTT500.00719622927.99999835
GGATTCT500.00719622927.9999986