Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n02_adr3wtzt14s37.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 198492 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 717 | 0.3612236261411039 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 241 | 0.12141547266388569 | No Hit |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 215 | 0.1083167079781553 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 203 | 0.10227112427704894 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 200 | 0.10075972835177237 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCTTAG | 20 | 0.0069436063 | 52.5 | 1 |
TATAGAG | 35 | 0.0012532601 | 40.000004 | 5 |
AGAGACC | 35 | 0.0012532601 | 40.000004 | 8 |
TGTAGGG | 45 | 9.1744056E-5 | 38.88889 | 2 |
GTAGGGG | 60 | 1.2114911E-5 | 35.0 | 3 |
CTGTAGG | 55 | 2.9846013E-4 | 31.81818 | 1 |
TAAAGAA | 55 | 2.9846013E-4 | 31.81818 | 5 |
TCAGTTA | 45 | 0.004299959 | 31.111113 | 8 |
TAGGACT | 80 | 2.4550482E-6 | 30.625 | 4 |
AGGACTA | 70 | 3.47646E-5 | 30.000002 | 5 |
CTAGGAC | 70 | 3.47646E-5 | 30.000002 | 3 |
TTTCGGA | 95 | 2.804736E-7 | 29.473686 | 1 |
TTCGGAA | 95 | 2.804736E-7 | 29.473686 | 2 |
TCTGTGG | 85 | 3.9373335E-6 | 28.82353 | 8 |
CATATGT | 50 | 0.007196229 | 27.999998 | 4 |
GAGTAGG | 50 | 0.007196229 | 27.999998 | 1 |
AAGACTC | 50 | 0.007196229 | 27.999998 | 4 |
TAGCAGA | 50 | 0.007196229 | 27.999998 | 4 |
GACATTT | 50 | 0.007196229 | 27.999998 | 35 |
GGATTCT | 50 | 0.007196229 | 27.999998 | 6 |