FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203103
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5320.2619360619980995No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2270.11176595126610636No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2190.107827063115759No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2110.10388817496541163No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATTA200.006943840552.52
AAGTAAA450.00430019431.11111368
TATGCGT450.00430019431.11111367
TTAGTTA450.00430019431.11111330
CATATAA853.93793E-628.823531
CGAAATC500.007196618228.00000251
AGTAAAC500.007196618228.00000269
ATATAAG1056.7306064E-726.6666682
AATACGC1101.0100175E-625.45454421
TTCTCCA700.001223080125.010
TATTTAG901.9212754E-423.3333342
ACCGGTA901.9212754E-423.33333415
ACTCCAC901.9212754E-423.33333410
GTCTTAC750.001827863623.3333341
GTAAGAC901.9212754E-423.3333343
TAAGAAC901.9212754E-423.3333345
CCCTATG952.7704999E-422.1052632
AACTCCA952.7704999E-422.1052639
TATAAGA952.7704999E-422.1052633
GCGCGTA800.002659666821.87520
Produced by FastQC (version 0.11.2)