FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163858
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6710.4095009093239268No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2670.16294596540907372No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA1990.12144661841350438No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1850.11290263520853422No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG1790.10924092812068986No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1770.10802035909140842No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC1720.1049689365182048No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA1710.10435865200356406No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGGT150.002219261870.017714
GTGTCAA150.002221951569.9963421
ACCGGTA350.001252942139.99790615
GACCACA400.002410407935.00885446
TAATAGT501.7046242E-434.9981768
TCACATA400.002414022634.9981761
CAAAGTA601.2106346E-534.9981763
GCTTTAA552.9832558E-431.81651941
ATATAAG450.00429246631.1189822
GGAGCGT450.00429888731.1094846
ACTCCAC450.00429888731.10948410
CATTATC703.4667937E-530.0075872
AAGTAAT604.967398E-429.16514265
AATACGC604.967398E-429.16514221
CTAATTT604.967398E-429.16514253
TTAAGCA755.561277E-527.99853344
AAAGTAG657.9325E-426.9216676
AAAGTAA657.9325E-426.92166764
CCGGTAA657.9325E-426.92166716
AGTAATA657.9325E-426.92166766