FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248883
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17250.6930967563071805No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT2560.10285957658819607No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2530.10165419092505314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG150.00222285170.04
CTCCTAG200.006945669752.530
CTATTAG350.001253874340.01
CGATCTG10700.036.9626165
GTATTAT400.002415804735.01
TTGGGAC601.21272715E-535.04
ATCTGAG1102.3646862E-1135.07
TTCCGAT24000.033.3958362
TGGGACC652.0974434E-532.3076935
CTTCCGA24600.032.296751
CCGATCT24250.032.1855664
TCCGATC24800.032.036293
TAGGAGC552.9867672E-431.818184
GATCTGG5250.031.3333346
ACCGGTA450.0043020431.11110915
TCTGTAC450.0043020431.1111093
TATAAGA450.0043020431.1111093
ATCTGGG2700.028.5185187
TCTTGTA1502.910383E-1128.0000022
GTTAACG500.007199686528.00000239
Produced by FastQC (version 0.11.2)