FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108005
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2500.23147076524234989No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2400.22221193463265587No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2070.1916577936206657No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA1830.1694366001574001No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1750.16202953566964493No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1700.15740012036479795No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA1700.15740012036479795No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC1620.14999305587704273No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC1530.14166010832831813No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG1490.13795657608444054No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA1460.13517892690153235No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT1310.12129068098699135No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC1280.11851303180408315No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT1240.11480949956020556No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1220.11295773343826675No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC1120.10369890282857276No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA1110.10277301976760335No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG1100.10184713670663395No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA1100.10184713670663395No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC1090.10092125364566457No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATTA200.006925642552.51806343
TCTGTTT200.006925642552.51806329
TTTTGGC200.006925642552.5180632
TATGTAC200.006925642552.5180633
ACTATGC200.006938345352.493759
CCGATTT200.006938345352.4937555
CACAGTG200.006938345352.4937550
TATCCAA305.8457104E-446.68272426
AAGGCTC350.001251456839.9952470
AGAACCT400.002405705435.01204329
AAAAGGC400.002411180934.99583468
GTCGTAA652.0882286E-532.3038534
TTAAGCG450.004284137431.12181548
TTTATGT450.004284137431.1218154
TAAGCGA450.004284137431.12181549
AAAGTGA450.00429386531.10740755
GTGTAGA450.00429386531.10740770
AAATTCT450.00429386531.10740721
AATTCTT450.00429386531.10740722
ACCGTGC450.00429386531.1074078
Produced by FastQC (version 0.11.2)